Myrosinase from Horseradish
Horseradish (Armoracia rusticana), is a perennial herbaceous horseradish belonging to the cruciferous family. The root of horseradish is edible, Most of the commercial crop is crushed fresh into sauces or used as a food additive for its pungent flavor. And due to its pungent taste, pests and diseases rarely occur. Myrosinase (EC126.96.36.199), also known as β-glucosinolate hydrolase, exists in the seeds, roots, stems and leaves of all glucosinolate-containing plants in cruciferous plants. It is a kind of enzyme to hydrolyze glucosinolate .
China is rich in horseradish resources, and there are abundant glucosinolates in horseradish. The degradation product isothiocyanate obtained under the catalysis of myrosinase has insecticidal and bactericidal effects, and can be used as a natural food bacteriostatic agent. Strengthening the research and utilization of horseradish myrosinase can not only increase the economic value of horseradish, but also provide excellent solid myrosinase preparation.
In 1995, Jwannyd et al. isolated two types of myrosinase from horseradish. It can be seen from the acetone extraction experiment on the crude enzyme solution from cabbage, radish and horseradish that the specific activity of myrosinase derived from horseradish is higher under the same conditions.
Myrosinase activtity of the four tested vegetables （Radish, Brussels sprouts, Watercress, Horseradish）using sinigrin or gluconasturtiin as substrate, measured by the proposed CE method and pH stat assay. All the results showed that the activity of myrosinase from horseradish was higher than the others .
 Jwanny E W , El-Sayed S T , Rashad M M , et al. Myrosinase from roots of Raphanus sativus[J]. Phytochemistry, 1995, 39(6):1301-1303.
Fang Qiang, et al.Study on Extracting Myrosinase from Different Sources by Acetone Precipitation. Preservation and Processing, 2008, 8(4):4.)
 Gonda, Sándor, et al. "Myrosinase Compatible Simultaneous Determination of Glucosinolates and Allyl Isothiocyanate by Capillary Electrophoresis Micellar Electrokinetic Chromatography (CE‐MEKC)." Phytochemical Analysis 27(2016).